Abstract
Background: Bowel preparation is necessary for successful colonoscopy, while it can seriously affect intestinal microbial composition and damage the intestinal mucosal barriers in humans.Methods: To figure out whether probiotics can sustain intestinal homeostasis and guard people's health, the probiotic drug of Bifidobacterium Tetragenous viable Bacteria Tablets (P group, n = 16) or placebo (C group, n = 16) was used for volunteers receiving bowel preparation, and high-throughput sequencing method was applied to monitor their intestinal microbial changes.Results: The present results suggested that bowel preparation obviously reduced the intestinal microbial diversity, while taking probiotics significantly restored it to normal level. In addition, probiotics sharply reduced the abundance of pathogenic Proteobacteria, and obviously lowered the ratio of Firmicutes/Bacteroidetes compared with control group at phylum level (P < 0.05). And probiotics markedly decreased the abundance of pathogenic Acinetobacter and Streptococcus, while greatly enriched the relative abundance of beneficial bacteria Bacteroides, Roseburia, Faecalibacterium, and Parabacteroides at genus level (P < 0.05).Conclusion: Probiotic drugs, e.g., Bifidobacterium Tetragenous viable Bacteria Tablets, can be used to restore intestinal dysbacteriosis caused by bowel preparation, and reduce side effects during colonoscopy.
Highlights
Colonoscopy is the preferred method to evaluate the intestinal health of most patients and it is the gold standard for colorectal cancer diagnosis, which can clearly discover intestinal lesions [1]
There was no significant difference between C group and P group
The results revealed that bowel preparation increased the relative abundance of Proteobacteria (0.266 vs. 0.410) while decreased the relative abundance of Actinobacteria (0.044 vs. 0.029), and had slightly effect on the relative abundance of Firmicutes (0.462 vs. 0.408) and Bacteroidetes (0.194 vs. 0.136) compared with the CB group
Summary
Colonoscopy is the preferred method to evaluate the intestinal health of most patients and it is the gold standard for colorectal cancer diagnosis, which can clearly discover intestinal lesions [1]. Symbiotic physiological anaerobic bacteria, symbiotic conditional pathogenic bacteria and other harmful bacteria co-exist in the intestinal tract in a stable proportion. During the bowel preparation procedure, large quantity of liquid enters the intestinal tract and considerably disrupts the environment of the normal intestinal cavity, and taking laxatives can enhance intestinal dyskinesia and intestinal peristalsis, which makes bacteria cannot adhere to intestinal mucosa [5]. The large amount of oxygen brought by bowel preparation in intestinal environment heavily reduces the number of anaerobic bacteria, and promotes the growth of aerobic bacteria, resulting in intestinal microbial disorder [6]. Bowel preparation is necessary for successful colonoscopy, while it can seriously affect intestinal microbial composition and damage the intestinal mucosal barriers in humans
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