Oral pre-administration of Purslane polysaccharides enhance immune responses to inactivated foot-and-mouth disease vaccine in mice

Rui Zhao,Bocui Song,Yongzhong Yu,Guiyan Jia,Xiangyu Meng

doi:10.1186/s12917-019-1782-3

Abstract

BackgroundFoot-and-mouth disease (FMD) is one of the greatest disease threats to animal husbandry worldwide. Though various vaccines against foot-and-mouth disease virus (FMDV) have been developed, vaccine effectiveness is still not satisfactory. In this work, we studied the potential ability of Purslane polysaccharide (POL-P3b) as a nutrient food additive to enhance immune responses to FMD vaccination in mice.ResultsOur results demonstrated that oral administration of POL-P3b at mid- and high-doses significantly enhanced the FMDV-specific cellular and humoral immune responses in mice and increased the concentration of Ca2+ in lymphocytes. Importantly, POL-P3b could promote intestinal DC maturation and stimulate the secretion of intestinal SIgA in a dose-dependent manner. Moreover, the acute toxicity study showed that POL-P3b was non-toxic and safe in mice.ConclusionOur findings provided solid evidence that POL-P3b might be a novel immunostimulator and a boosting agent for increasing the efficacy of FMD vaccination, and the mechanism was related to stimulating the intestinal mucosal immune function that subsequently enhanced the efficacy of FMD vaccination through pre-administration of oral POL-P3b.

Highlights

Foot-and-mouth disease (FMD) is one of the greatest disease threats to animal husbandry worldwide
Foot-and-mouth disease (FMD) is a common infectious disease of cloven-hoofed animal caused by the foot-and-mouth disease virus (FMDV), which is listed as a notifiable animal disease by the World Organization for Animal Health (OIE)
Compared to mice in control group, a significant IgG response was found in mice immunized with FMD vaccines (Fig. 1)

Summary

Introduction

Foot-and-mouth disease (FMD) is one of the greatest disease threats to animal husbandry worldwide. Though various vaccines against foot-and-mouth disease virus (FMDV) have been developed, vaccine effectiveness is still not satisfactory. Foot-and-mouth disease (FMD) is a common infectious disease of cloven-hoofed animal caused by the foot-and-mouth disease virus (FMDV), which is listed as a notifiable animal disease by the World Organization for Animal Health (OIE). At present, both the O and A type of FMD are commonly found in China and pose a great threat to the development of animal husbandry through various routes of transmission.

Methods

Results

Conclusion