Oral mucosa transplantation may improve tear film osmolarity in dogs with keratoconjunctivitis sicca - a preliminary study

F.L.C Brito,T.O.C Marinho,J.N Voitena,A Kuner

doi:10.1590/1678-4162-12421

F.L.C Brito, T.O.C Marinho + Show 2 more

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https://doi.org/10.1590/1678-4162-12421

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Abstract

ABSTRACT The objective of this study was to evaluate the use of cyclosporine 1% alone or associated with oral mucosa transplantation (OMT) in dogs with dry keratoconjunctivitis (KCS). Schirmer Tear Test (STT-1) and Tear Film Osmolarity (TFO) were measured in both eyes of 30 adult dogs (before and 45 days after treatment. The animals were divided into three groups (10 dogs for group): control (normal dogs), group I (GI, treated with 1% cyclosporine alone), and group II (GII, treated with 1% cyclosporine and OMT). All STT-1 and TFO values were subjected to the Shapiro-Wilk normality test, and all were normally distributed. STT-1 and TFO values before and after treatment were subjected to the T-Student Test. The STT‐1 and TFO values of the right eye were subjected to Repeated Measures ANOVA followed by a Tukey Test for comparison between groups I and II. Means with a value of p≤0.05 were considered significant. There was a decreased osmolarity in both groups after treatment. Mean osmolarity in GII (322.60±16.56 mOsm/L) was significantly lower than GI (336.40±5.66 mOsm/L). The OMT associated with cyclosporine 1% improved the osmolarity of the tear film in dogs with KCS with a seeming synergism between the clinical and surgical treatments.

Highlights

Keratoconjunctivitis sicca (KCS) is commonly diagnosed in canine patients and has an extensive list of potential etiologies, the most frequent cause being immune-mediated (Kaswan et al, 1998; Williams, 2008; Giuliano, 2013)
All s Tear Test-1 (STT-1) and Tear film osmolarity (TFO) values were subjected to the Shapiro-Wilk normality test, and all were normally distributed
This study describes the results of KCS treatment in dogs with 1% cyclosporine alone or associated with OMT

Summary

Introduction

Keratoconjunctivitis sicca (KCS) is commonly diagnosed in canine patients and has an extensive list of potential etiologies, the most frequent cause being immune-mediated (Kaswan et al, 1998; Williams, 2008; Giuliano, 2013). Hyperosmolarity evaluation is the gold standard for the diagnosis of dry eye in humans, in addition to being recognized as a proinflammatory stimulus in the development of the disease (Bron et al, 2017). The measurement of film lacrimal osmolarity has often been reported as the best method to diagnose dry eye and determine disease severity (Sullivan et al, 2010). It is considered an important diagnostic test in human medicine, (Lemp et al, 2011; Potvin et al, 2015) in veterinary medicine, the data are still incipient, and the test has not been implemented in clinical routine

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