Oral Microbiocenosis in Cases of Inflammatory Periodontal Diseases in Children with Tuberculosis

Abstract

Objective — to examine the microbial spectrum of the oral cavity in cases of inflammatory periodontal diseases of children with tuberculosis. Materials and methods. A study of the microbial habitat of oral biotopes and gingival surfaces was conducted in 97 children aged 6 to 17 years with inflammatory periodontal diseases and tuberculosis (treatment group). The control group consisted of 20 practically healthy children of the same age. The periodontal diseases were diagnosed according to M.F. Danilevsky’s classification (2000). The study of oral microbiocenosis and gingival surfaces in the examined groups of children was performed in accordance with Order No. 234 of the Ministry of Health of Ukraine as of 2006. Results and discussion. Aerobic, facultative anaerobic, and anaerobic bacteria persisted in the oral fluid and on the gingival surfaces of children with tuberculosis and inflammatory periodontal diseases. The most frequent colonies of bacteria were Streptococcus, in particular S. mutans, S. salivarius, S. β-haemolyticus, Staphylococcus (S. aureus, S. epidermidis).The significant evidence of Neisseria (N. catarrhalis) and Corynebacterium (C. xerosis) was revealed in the oral cavity and gingival surfaces of the examined children. The microflora of children from the treatment group revealed mostly Lactobacillus genus. The microbial associations of the examined children consisted of Candida, E. coli fungi. Smear microscopy revealed viruses. S. aureus was higher in the children from the treatment group as compared to the healthy children. In addition, the concentration of microorganisms was higher on the gingival surface versus the oral fluid and was more evident in the children from the treatment group. Conclusions. The obtained spectrum of the oral microbial contamination in the children with tuberculosis and inflammatory periodontal diseases revealed the persisting associations of aerobic, facultative anaerobic, and anaerobic bacteria. The inflammatory periodontal diseases in tuberculosis-infected children were accompanied by the evident increase in the frequency and concentration of microorganisms increasing with the higher severity of the specific process.