Abstract
Nanoparticles have shown a certain potential to overcome thedrawbacks of oral peptide delivery in the gastrointestinal tract such as lowpeptide stability and permeability. Insulin PLGA NP were prepared using amodified double emulsion solvent evaporation technique. Insulin PLGA NP were composedfrom human insulin (5 mg) encapsulated in PLGA 2.5% (w/v) mixed with PEG (2kDa, 5% w/w) and the external aqueous phase contained 1.25% of PVA. Theresulting nanoparticles of 202.6 nm diameter and loaded with 33.86 μg insulinper mg of polymer were utilised in this study to examine the hypoglycaemiceffect after combination with a protease inhibitor, N-Ethylmaleimide.
Highlights
SUMMARYNanoparticles (NP) have shown a certain potential to overcome the drawbacks of oral peptide delivery in the gastrointestinal tract such as low peptide stability and permeability
Insulin is sensitively degraded by proteolytic enzymes in the gastrointestinal tract
To investigate the effect of protease inhibitor (PI), two other animal groups of rats were utilised for free insulin and insulin PLGA-NP without
Summary
Nanoparticles (NP) have shown a certain potential to overcome the drawbacks of oral peptide delivery in the gastrointestinal tract such as low peptide stability and permeability. Insulin PLGA NP were prepared using a modified double emulsion solvent evaporation technique. Insulin PLGA NP were composed from human insulin (5 mg) encapsulated in PLGA 2.5% w/v mixed with PEG (2 kDa, 5% w/w) and the external aqueous phase contained 1.25% of PVA. The resulting nanoparticles of 202.6 nm diameter and loaded with 33.86 μg insulin per mg of polymer were utilised in this study to examine the hypoglycaemic effect after combination with a protease inhibitor, N-Ethylmaleimide
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