Abstract
β -Thalassaemia [thalassaemia major] causes severe transfusion dependent anemia. In the heterozygous state, the β Thalassaemia trait [thalassaemia minor] causes mild to moderate microcytic anemia. Our study comprised of hundred β Thalassaemia major patients undergoing a minimum of 15 transfusions and hundred normal individuals. Both the control and study group excluded patients with iron deficiency anemia, megaloblastic anemia, malignancy and chronic liver damage. Exfoliated cells from the buccal mucosa of 65 out of the 100 β -Thalassaemia major patients revealed positivity for Perl’s Prussian blue reaction. Applying the chi-square test to compute the p-value, from the computations it is evident that p 0.005. Hence, we concluded that Perl’s Prussian blue reaction test is not gender based.
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