Abstract
Our previous study showed that vaccination with plasmid DNA containing infectious bursal disease virus (IBDV) gene which encodes complete polyprotein (VP2/4/3) induced protective immune responses. In this study, we examined the efficacy of an oral DNA vaccine carrying the IBDV polyprotein antigen delivered by attenuated Salmonella enterica sv. Typhimurium ( S. typhimurium). The recombinant plasmid pCI-VP2/4/3 was transformed by electroporation into an attenuated S. typhimurium strain (Dam ̄ PhoP ̄) (designated hereafter as SV/pCI-VP2/4/3). The IBDV polyprotein gene was expressed in chicken embryo fibroblast (CEF) cells infected with strain SV/pCI-VP2/4/3, as shown by gene-specific RT-PCR and Western blot. Oral immunization of 7-day-old specific-pathogen-free (SPF) chickens with SV/pCI-VP2/4/3 elicited specific humoral responses as measured by ELISA. Vaccination with the strain SV/pCI-VP2/4/3 at 10 9 CFU per chicken offered 11/15 (73.3%) protection of the chickens against virulent IBDV challenge. Our results have implications in the development of DNA vaccines against avian viral diseases by bacteria-vectored oral delivery system.
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