Abstract
Oral bacterial biofilms – history in progress
Highlights
Ask microbiologists what the word ‘biofilm’ brings to mind, and many would speak about Pseudomonas aeruginosa, about microtitre plates or flowcells, about ‘mushrooms’ and ‘voids’, and about antibiotic resistance and device-related infections
The biofilms were retrieved at sequential time points on small pieces of glass or bovine enamel that had been carried in the oral cavity of a volunteer: typically an eager(?) student
Carrier pieces were simultaneously examined with the electron microscope
Summary
Ask microbiologists what the word ‘biofilm’ brings to mind, and many would speak about Pseudomonas aeruginosa, about microtitre plates or flowcells, about ‘mushrooms’ and ‘voids’, and about antibiotic resistance and device-related infections. Count, and identify organisms after removal, the biofilm was scraped off, homogenized, and plated on various media. On (¡4 h of carrier wear), the biofilm consisted of small aggregates of spherical cells (generally no more than three or four cells) with the occasional non-coccoid morphotype thrown in.
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