Abstract
Insufficient detoxification and/or overproduction of reactive oxygen species (ROS) induce cellular and tissue damage, and generated reactive oxygen metabolites become exacerbating factors of dermatitis. Keishibukuryogan-ka-yokuinin (KBGY) is a traditional Japanese medicine prescribed to treat dermatitis such as acne vulgaris. Our aim was to verify the antioxidant properties of KBGY, and identify its active constituents by blood pharmacokinetic techniques. Chemical constituents were quantified in extracts of KBGY, crude components, and the plasma of rats treated with a single oral administration of KBGY. Twenty-three KBGY compounds were detected in plasma, including gallic acid, prunasin, paeoniflorin, and azelaic acid, which have been reported to be effective for inflammation. KBGY decreased level of the diacron-reactive oxygen metabolites (d-ROMs) in plasma. ROS-scavenging and lipid hydroperoxide (LPO) generation assays revealed that gallic acid, 3-O-methylgallic acid, (+)-catechin, and lariciresinol possess strong antioxidant activities. Gallic acid was active at a similar concentration to the maximum plasma concentration, therefore, our findings indicate that gallic acid is an important active constituent contributing to the antioxidant effects of KBGY. KBGY and its active constituents may improve redox imbalances induced by oxidative stress as an optional treatment for skin diseases.
Highlights
Skin is chronically exposed to endogenous and environmental stimuli, leading to the harmful generation of reactive oxygen species (ROS) [1,2]
We focused on the antioxidant potential of KBGY for decreasing reactive oxygen metabolites related to the pathogenesis and progression of skin diseases
This study revealed that 39 constituents were identified in KBGY
Summary
Skin is chronically exposed to endogenous and environmental stimuli, leading to the harmful generation of reactive oxygen species (ROS) [1,2]. In patients with acute exacerbation of atopic dermatitis, 8-hydroxy-deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adducts (markers of lipid peroxidation), and bilirubin oxidative metabolites (markers of bilirubin oxidative metabolism) are demonstrated to be increased significantly in urine and return to normal levels during disease remission [8,9]. Some of the reactive oxygen metabolites can circulate in the blood due to their relative stability compared to ROS, and can become exacerbating factors of systemic and/or prolonged inflammation. It is of importance in diagnosis and treatment for refractory dermatosis to understand the mutual relationship among peripheral blood flow, reactive oxygen products, and inflammation
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