Oral administration of Lactobacillus gasseri SBT2055 is effective for preventing influenza in mice

Hisako Nakagawa,Takuya Shiozaki,Fumihiko Sakai,Yosuke Nakayama,Noriko Ikeda,Tomohiro Moriya,Tomohiro Hosoya,Tadaaki Miyazaki

doi:10.1038/srep04638

Hisako Nakagawa, Takuya Shiozaki + Show 6 more

Open Access

https://doi.org/10.1038/srep04638

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Abstract

The Lactobacillus gasseri SBT2055 (LG2055) is a probiotic lactic acid bacterium with properties such as bile tolerance and ability to improve the intestinal environment. In this study, we established that the oral administration of LG2055 exhibits efficacy to protect mice infected with the influenza virus A/PR8. The body weight losses were lower with the LG2055 administration after the PR8 virus infection. At 5 days after the infection, the virus titer was significantly decreased as was the amount of produced IL-6 in the lung tissue, the number of total cells in the bronchoalveolar lavage fluid was reduced by the LG2055 administration. The expression of the Mx1 and Oas1a genes, critical for the viral clearance in the lung tissues was increased by the pre-treatment with LG2055. These findings suggest that the LG2055 administration is effective for the protection against influenza A virus infection by the down-regulation of viral replication through the induction of antiviral genes expression.

Highlights

The Lactobacillus gasseri SBT2055 (LG2055) is a probiotic lactic acid bacterium with properties such as bile tolerance and ability to improve the intestinal environment
The oral administration of LG2055 protects the mice from a lethal Puerto Rico/8/34 (PR8) virus infection, and the virus titer in the bronchoalveolar lavage (BAL) fluid is significantly decreased by LG2055 administration at 5 days after the virus infection
The LG2055 solutions were administered at two concentration levels (1.0 3 109 or 1.0 3 108 cfu/200 ml), and the survival rates of the mice orally administered with these LG2055 solutions after the PR8 virus infection were monitored

Summary

Introduction

The Lactobacillus gasseri SBT2055 (LG2055) is a probiotic lactic acid bacterium with properties such as bile tolerance and ability to improve the intestinal environment. The Lactobacillus gasseri SBT2055 (LG2055) is a probiotic lactic acid bacterium isolated from human feces with properties such as bile tolerance, ability to become established in the intestine and to improve the intestinal environment, and it has preventive effects on abdominal adiposity in rats and humans[2,3,4,5]. This bacterium is chosen as a probiotic strain due to its suitability as a starter for preparing fermented milk. These results indicate that the oral administration of LG2055 is efficient for the prevention of influenza by the inhibition of virus replication via up-regulation of the expression of antiviral genes such as Mx1

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