Oral Administration of Bovine and Porcine Milk Exosome Alter miRNAs Profiles in Piglet Serum

Delin Lin,Qianyun Xi,Yongliang Zhang,Meiying Xie,Ruiping Sun,Qingyan Jiang,Dingze Ye,Yanling Zhu,Meng Li,Jiahan Wu,Bin Zeng,Jiajie Sun,Ting Chen

doi:10.1038/s41598-020-63485-8

Delin Lin, Qianyun Xi + Show 11 more

Open Access

https://doi.org/10.1038/s41598-020-63485-8

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Abstract

Breast milk is the most important nutrient source for newborn mammals. Studies have reported that milk contains microRNAs (miRNAs), which are potential regulatory components. Currently, existing functional and nutritional two competing hypotheses in milk field though little date have been provided for nutritional hypothesis. In this study, we used the qRT-PCR method to evaluated whether milk miRNAs can be absorbed by newborn piglets by feeding them porcine or bovine milk. The result showed that miRNA levels (miR-2284×, 2291, 7134, 1343, 500, 223) were significantly different between bovine and porcine milk. Four miRNAs (miR-2284×, 2291, 7134, 1343) were significantly different in piglet serum after feeding porcine or bovine milk. After separated milk exosomes by ultracentrifugation, the results showed the selected milk miRNAs (miR-2284×, 2291, 7134, 1343) were present in both exosomes and supernatants, and the miRNAs showed the coincidental expression in IPEC-J2 cells. All our founding suggested that the milk miRNAs can be absorbed both in vivo and in vitro, which will building the foundation for understanding whether these sort of miRNAs exert physiological functions after being absorbed and provided additional evidence for the nutritional hypotheses.

Highlights

Breast milk is the first and most important source of nutrition for newborn mammals[1]
The results showed that miR-2284×and miR-2291 level were remarkably higher in the bovine milk-feeding group than in the porcine milk-feeding group on day 6 and day 12 and no difference expression on day 0 and 3 (Fig. 2A-B)
There were no significant differences in the levels of miR-500 and miR-223 between the two groups (Fig. 2E–F). These results were coincidental with the corresponding miRNA levels in bovine and porcine milk whey (Fig. 1). These results indicated that milk-derived miRNAs can be absorbed by newborn piglets and exhibited different content profiles among days, that maybe relevant to diverse physiological requirement after birth

Summary

Introduction

Breast milk is the first and most important source of nutrition for newborn mammals[1]. Studies have reported that 12 body fluids contain miRNAs, and milk has the highest concentration of total RNA that is rich in miRNAs21. Chen et al reported the presence of 245 miRNAs in bovine milk[27], and Kosaka et al detected 281 of 723 known human miRNAs in human milk by microarray technology[28]. Wolf et al reported that miRNAs in bovine milk are transferred among animal species by dietary means because bovine milk exosomes can be absorbed by human and rat intestinal cells[49]. To evaluate whether milk-derived miRNA is absorbed in newborn piglets, we used bovine and porcine milk, which have different miRNA expression profiles, for in vivo and in vitro experiments. This study will provide evidence on miRNA absorption in newborn mammals

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