OR67. The Effects of Electric Cigarette Smoke and Conventional Cigarette Smoke Exposure on the Increase of Atherogenic Marker: Leptin Receptor (mRNA-ObR) and Aortic Calcification in Mice

Abstract

Abstract Aims The effect of e-cigarettes on atherosclerosis is still a matter of debate. One method that can be used as atherogenic process marker is the expression of Leptin receptors (mRNA-ObR) and aortic calcification. The aim of this study was to compare Leptin receptors (mRNA-ObR) expression and aortic calcification in mice exposed to conventional cigarettes and e-cigarettes. Methods and Results Thirty samples of mice were randomly divided into control group, conventional cigarette exposure group, and e-cigarette exposure group. After 2 months of treatment using a modified smoking chamber, a surgical procedure was performed to remove aortic tissue. This procedure was followed by histopathological examination to discover aortic calcification, and RT-PCR examination to see the expression of mRNA-ObR. The results of post hoc analysis showed that control group and conventional cigarette exposure group had differences in mRNA-ObR expression and aortic calcification [0.030 (0.090-0.046) fg/µl vs 0.332 (0.990-0.537) fg/µl, p = 0.000, in mRNA ObR; and 0.130 ± 0.719% vs 1.678 ± 0.696%, p = 0.000, in aortic calcification], as well as control group and e-cigarette exposure group [0.030 (0.090-0.046) fg/µl vs 0.376 (0.218-0.464) fg/µl, p = 0.000, in mRNA ObR and 0.130±0.719% vs 1.493±0.496%, p = 0.000, in aortic calcification]. Meanwhile, the group with e-cigarette exposure and group with conventional cigarette exposure didn’t show any differences [0.376 (0.218-0.464) fg/µl vs 0.332 (0.990-0.537) fg/µl, p = 0.568, in mRNA ObR and 1.493 ± 0.496% vs 1.678 ± 0.696%, p = 0.889, in aortic calcification]. Conclusion There were no differences in Leptin receptor (mRNA-ObR) expression and aortic calcification in conventional cigarettes and e-cigarettes exposure groups.