OR15. Cell Surface Markers on Adipose-Derived Mesenchymal Stem Cells: Isolation, Culture, and Identification for Translational Success

Abstract

Abstract Aims The purpose of this study is determining that each part of adipose tissue will generate stem cells with similar adipose-derived mesenchymal stem cells (AMSCs) characteristic and specific cell-surface markers, thus there is no waster product of part adipose tissue in regenerative medicine. Methods An invitro experimental study, used approximately 20 grams of human subcutaneous adipose tissue were isolated and processed, until stromal vascular factor (SVF) and creamy supernatant separated. Both substances then divided into single filtration group and double filtration group consecutively. Each group and control group were sextuplicate before further analysis using immunocytochemistry. Single and double filter of AMSCs were applied to the subsequent experiments. AMSCs were observed under a microscope, the growth curves of the cells were assessed using a cell counting kit‑8 assay and the membrane expression of cell surface antigens, including cluster of differentiation (CD) 34, CD45, CD90, and CD105 were detected by immunocytochemistry. Results Flow cytometry demonstrated that the cells were highly positive for fourth cell surface markers. CD34 and CD45 expressions were detected in control group, and gradually decreased among another groups whereas lack of CD45 expression were consistent on other remaining groups. The expression of CD90 and CD105 were visibly increased significantly from control group to fourth group. Conclusion This study demonstrated that either SVF and/or supernatant generates fibroblastic-like cells and surface markers expression that matched the MSCs criteria. Therefore, all parts of adipose tissue are an appealing alternative of stem cell source regardless of culture method chosen.