OR05-4 Positive association of serum prolactin concentrations with all-cause and cardiovascular mortality: Results from a 10-year follow-up of the Study of Health Pomerania (SHIP)

Abstract

of ECM deposition in bGH mice. ECM deposition in adipose tissue was assessed in WAT collected from bGH versus littermate control mice at 10, 26, and 62 weeks of age, in both males and females. Adipose tissue samples from subcutaneous, perigonadal, retroperitoneal, and mesenteric depots were fixed and embedded in paraffin blocks and stained with pico-sirius red. When assessing overall collagen content, the bGH mice showed dramatic increases in ECM deposition when compared to the WT controls at most ages and in both sexes. When comparing different depots, collagen staining appeared most prevalent in the subcutaneous depot as compared to intra-abdominal depots in both male and female mice. Based on these results, analysis of collagen gene expression levels will be conducted. Specifically, collagens I, III, IV, V and VI in the subcutaneous, epididymal, mesenteric, and retroperitoneal adipose tissue depots of 6 month-old male mice will be examined through quantitative PCR analysis and results will be reported. Our histological results demonstrate a novel role of GH in adipose tissue function and suggest that ECM deposition likely contributes to the WAT depot specific differences previously reported in mice with modified GH action. Additional studies will be conducted in other GH transgenic mouse models to further establish the connection between GH and the collagen content of WAT and to deepen our understanding of the role of the ECM in WAT dysfunction.