Optofluidics biosensor based on speckle focusing

Abstract

Biography Aoqun Jian received the B.Eng. degree in electronic and information technology and the M.Eng. degree in microelectronics and solid electronics from the North University of China, Taiyuan, China, in 2005 and 2008, respectively. He received the Ph.D. degree in the Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong, in 2013. He is currently an associate professor with College of Information Engineering, Taiyuan University of Technology. He has published more than 10 journal papers and has co-authored two books. His current research interests include optofluidics, photonics and sensing devices.   Optofluidics biosensor based on speckle focusing Although the lab-on-a-chip system has been successfully applied in a wide variety of fields, a biosensor which has a simple structure, low cost and high flux still attracts continuous research efforts. Here, the authors explore a biosensor based on the speckle focusing to detect the concentration of the T lymphocyte. Firstly, several optical fibers are embedded in the trench fabricated previously by dry etching in the silicon substrate as the incident light source. The gold pattern with lens shape obtained by typical lift-off technology is modified with the anti-CD3 which can specific bind the T lymphocyte suspended in the solution. The light emits from the optical fibers are collimated by the collimating lens formed in the polydimethylsiloxane (PDMS). The T lymphocyte bound on the gold pattern acts as a convex lens，focusing the collimated light propagates through it. The focused light is collected by an optical fiber embedded at the other side of the device, and the intensity of the light indicates the concentrations of the T lymphocyte. This sensor is particularly useful for drug screening, cell pathology analysis and cancer pre-diagnosis.   Acknowledgement This work was financially supported by National Natural Science Foundation of China (No. 61501316), Taiyuan University of Technology, Basic Research Program of Shanxi for Youths (No. 2012021013-1), The Shanxi Provincial Foundation for Returned Scholars (2015-047) and 863 project (2015AA042601).