Abstract
An improved ion-exchange chromatographic method for rapid separation and quantitative estimation of deoxyfructosyllysines and their hydrolysis products (pyridosine and furosine) is described. The proposed elution procedure has good reproducibility; the optimum hydrolysis conditions for the highest molar ratio of pyridosine and furosine are given. The furosine/pyridosine molar ratios obtained by ⌈dhydrolysis” of model fructosyllysine as well as of natural protein matrices (soya beans, tomato powder, barley and malts) are in good agreement and without exception > 1. The smallest amount of pyridosine determined was 0.009% (w/w). The relationship between the compounds pyridosine, furosine, lysine and carbohydrate units present in the parent moiety is discussed.
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