Optimum Timing of an Application of Corn Oil and Bacillus thuringiensis to Control Lepidopteran Pests in Sweet Corn

Abstract

Corn oil and Bacillus thuringiensis ssp. kurstaki (Bt) applied directly into the silk channel of a corn ear has been shown to be an effective pesticide against corn earworm, Helicoverpa zea (CEW), and european corn borer, Ostrinia nubilalis (ECB). Field studies were conducted in 2000 and 2001 to determine the influence of application timing on ear quality at harvest. Two blocks of corn were planted during each year to observe treatment effects under varying populations of the two insect species. The treatment consisted of 0.5 mL (0.017 floz) of food grade corn oil containing a suspension of Bt at 0.08 g (0.003 oz) a.i. per ear applied directly into the silk channel at the husk opening. One treatment application was made on each silk day 3 through 11 from first silk; silk day 1 was the first day that 50% or more of ears had 2.5 cm (1 inch) of silk protruding from the husk. One treatment did not receive the oil + Bt suspension. All ears were harvested at milk stage, on silk day 25. The number of CEW larvae in treated ears increased with later application days in 2000, but not in 2001. Damage from larval feeding was mainly found near the tip of the ear, and damage ratings were lower compared to untreated ears for all treatment days for both plantings in 2000, and through application day 8 in the late planting of 2001. ECB larvae were reduced for all treatment days in both plantings in 2000 and the late planting of 2001. The percentage of ears rated as marketable (i.e., free of feeding damage) ranged from 71% to 100% in treated plots compared to 30% to 77% in the untreated plots. There was a linear decrease in marketability with later application days in two of the four plantings. The greatest decrease in marketability was after application day 7. Because the oil application affects kernel development at the tip, the length of ear with under-developed kernels, or cone tip, was measured. The number of ears with cone tip decreased linearly with the later application days in all plantings. There was 10% conetip or less after day 7 in 2000 and day 6 in 2001. The best combination of effective insect control resulting in the highest rates of marketable ears with the least degree of cone tip was achieved in this experiment by application of oil + Bt suspension on day 7. Year to year variation in the environment would suggest a range from day 6 to 8.