Abstract
AbstractOptimum production of extracellular, thermostable amylolytic enzymes (α and β‐amylase) by a newly isolated bacterium, Bacillus stearothermophilus, was investigated in a batch bioreactor. Starch and lactose at 1.0% and 3.0% (w/v) respectively were found to be optimum for maximum enzyme production. Optimization of cultural conditions (pH 7.0 and temperature 45°C) resulted in high bacterial specific growth rate (0.64 h−1), yielding 2.20 gL−1 biomass, 11.43 UmL−1 α‐amylase and 10.04 UmL−1 of β‐amylase. Hydrolysis of native starches from wheat, cassava, corn and potato at 60°C using the crude enzyme showed 60‐80% saccharification with potato starch showing the least and wheat starch showing the greatest hydrolysis. The Km and Vmax values of the crude α‐amylase for starch were 4.78 mg starch/mL and 6.67 mg/mL.min respectively.
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