Abstract
The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.
Highlights
The reduction in the fossil fuel reserves and the constant increase in the consumption of energy stimulated the interest of research for the renewable sources of energies
The aim of the present work was to produce an immobilized lipase from Candida antartica B
Probability (p
Summary
The reduction in the fossil fuel reserves and the constant increase in the consumption of energy stimulated the interest of research for the renewable sources of energies. The biodiesel is one of the most promising alternatives. Among all the methods of production of biodiesel, the transesterification is most effective and most significant. It is about a process in which vegetable oils, animal fat or oils containing microalgae are mixed with an alcohol (ethanol or methanol) in the presence of a catalyst, chemical (acid or bases) or enzymatic [2]. The vegetables oils most used are mainly those of sunflower, colza, soya, Jatropha or palm [3]
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