Abstract
Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum. The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method. The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions. The molecular weight, polydispersity, and conformation of these fractions were also characterized. In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant. This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
Highlights
The genus Dendrobium belongs to the tribe Dendrobieae, the second largest genus in the family Orchidaceae with approximately 800–1500 species [1]
The extracts of each sample were cooled at room temperature and filtered, and the polysaccharide concentration of the Dendrobium devonianum flowers polysaccharides (DDFPs) was determined by the phenol–sulfuric acid method [11], with glucose solutions (50, 100, 150, and 200 μg/mL) as the standards
The Response surface methodology (RSM) optimization of the ultrasonic-assisted extraction (UAE) conditions was based on the maximum DDFP yield of the sample
Summary
The genus Dendrobium belongs to the tribe Dendrobieae, the second largest genus in the family Orchidaceae with approximately 800–1500 species [1]. The stems and leaves of D. officinale exhibit medicinal properties [2,3,4]. Because of overexploitation, the valuable D. officinale plant has been on the verge of extinction This herb is in high demand and has a high market value in China. Far, there are limited studies on the optimization of the extraction process of DDF polysaccharides (DDFPs) as well as their characterization and antioxidant activities. In this study, both UAE and RSM were used to optimize the extraction conditions of DDFPs based on a International Journal of Analytical Chemistry single-factor preliminary experiment. The aforementioned fractions were characterized by highperformance size-exclusion chromatography coupled with multiangle laser light scattering (HPSEC-MALLS) and highperformance anion-exchange chromatography coupled with pulsed amperometric detector (HPAEC-PAD) analysis
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