Abstract

Different nutritional and cultural factors were studied to determine the optimum conditions for prodigiosin production by Serratia marcescens S11 in a batch culture of brain-heart infusion broth medium. These factors include carbon source and its concentration, nitrogen source and its concentration, phosphate source, temperature and pH. Results showed that the optimum conditions for prodigiosin production were achieved when the production medium was supplemented with olive oil and casein hydrolysate as a carbon and nitrogen sources respectively in a concentration of 1.5% for broth, KH2PO4 as a phosphate source at initial medium pH8, and incubation at 28°C for 24 hours. Under these optimal conditions, prodigiosin activity produced by Serratia marcescens S11 in culture medium was increased from 200 U/cell before optimization to 3000 U/cell.

Highlights

  • Prodigiosin is a secondary metabolite produced by Serratia marcescens and many other grams negative and positive bacteria [1]

  • Microorganisms differ in their needs to carbon sources according to their nutrient nature; the use of pure carbon sources e.g. is expensive from the economical case, so the industrial fermentation try to use cheap carbon sources especially industrial and a variety of plant seed oils have been used as carbon substances for prodigiosin production and displayed stimulatory effects on the production by S. marcescens

  • Mineral salts have an effect on the production of prodigiosin and there are several studies which demonstrated that synthesis of prodigiosin by non-proliferating cells of S. marcescens is depends on the presence of inorganic phosphate (Pi) concentrations [6,7]

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Summary

Introduction

Prodigiosin is a secondary metabolite (red pigment) produced by Serratia marcescens and many other grams negative and positive bacteria [1]. It was of great interest in medicine due to its antifungal, antibacterial, antiprotozoal, antimalarial, immunosuppressive, and anticancer activities [2, 3]. Optimum growth of all strains of Serratia has been observed at a temperature from (20-37) ̊C, while the optimum temperature of prodigiosin production was at 30 ̊C [5]. Microorganisms differ in their needs to carbon sources according to their nutrient nature; the use of pure carbon sources e.g. According to the importance of prodigiosin in different applications, this study was aimed to determine the optimum conditions for prodigiosin production by local isolate of S. marcescens

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