Abstract
The GC-ECD was used in this study. The optimization of preparation was performed by U9(94)uniform design method, With the rate of 2,6-DNT -degrading result as investigative indexes, the influences of ratio the concentration of substrate and PH, The speed of shaker and Temperature .the result were screened by Uniform Design to found the optimum condition of degrading . Result the optimum condition for 2, 6-DNT -degrading as following: The concentration of substrate is 135mg/L, pH=7.3, Temperature is 29°C, speed of shaker is 129 rpm/min. The result show: It is scientific and feasible to screen the experimental condition of for 2，6-DNT - degrading by using the uniform design and regression.
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