Abstract
Abstract The optimization of chromatographic conditions for the determination of 2,6-di-t-butyl-U-pyrazinyl-aminophenol(DBAP) on reversed-phase HPLC with an aqueous-organic mobile phase were investigated. Undesirable peak shape, of which the quinone form(QF) was produced by the decomposition of DBAP in a stainless steel column, was observed on both of RPC packings containing small and large amounts of metal impurities. Although this conversion reaction was suppressed by washing the ODS gels with a 1 mM EDTA solution, the decomposition of DBAP in the column was still taking place. Concluding that the contamination of metal impurities on the column packing occurs continueously. The use of inert columns such as peek (polyether-ether-ketone), glass-lining columns but except a titan column, packed a high purity RPC packing material which was washed with the EDTA solution prior to use, led to the quantitative result of DBAP in the inert chromatographic system.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.