Abstract
Rosa canina L. (dog rose) is an important ornamental, edible and medicinal plant. It has been used as a rootstock for ornamental roses, grown in plantations for fruit harvesting and it is suitable for revegetation of abandoned mine lands. The propagation of native genotypes that are well adapted to local conditions can provide planting material for both revegetation and plantation purpose. Micropropagation is the most suitable method for a rapid vegatative propagation of selected wild genotypes, but an increased presence of pathogens as well as higher contamination rate during culture establishment were expected. An occurrence of a specific Fe-chlorosis during in vitro propagation of roses is also possible. Therefore, the optimal period and disinfection protocol for establishing sterile in vitro culture of selected genotypes of dog rose was investigated, as well as an effect of increasing the FeEDTA concentration in the MS medium during multiplication phase. The obtained results showed that the optimal time for taking initial explants corresponds to optimal time for taking green cuttings in traditional vegetative propagation by softwood cuttings, and the best results were achieved using shoots collected in the first week of May, when the flowers were open. The iron chelate concentration in the medium affected the mean number of shoots, and doubling of its concentration resulted in a considerably higher number of shoots per explant.
Highlights
Rosa canina L. (Rosaceae) is often used as a rootstock for ornamental roses, especially cultivars ‘Inermis’, ‘Pfänders’, ‘Schmids Ideal’ and ‘Laxa’, considered as adaptable to different types of soil (Ka ra s e k, 1976; M rat i n i ć, Ko j i ć, 1998; Deb en e r, Gudin, 2003).Recent studies showed that R. canina can be used for the revegetation of abandoned mine lands
Dog rose hips proved efficient against osteoarthritis and rheumatoid arthritis, especially with regard to its anti-inflammatory properties and their capacity to reduce pain (S e l a hva rzian et al, 2018; Christensen et al, 2008)
The presented research showed that the time of establishing in vitro culture, using shoots and axillary buds as initial explants, are important and they considerably affect the success of culture development
Summary
Recent studies showed that R. canina can be used for the revegetation of abandoned mine lands. Makineci et al (2011) investigated the plant species composition of naturally revegetated areas of abandoned open coal mines, and R. canina proved as one of the species that successfully adapted to unfavorable conditions in the researched area. Dog rose hips proved efficient against osteoarthritis and rheumatoid arthritis, especially with regard to its anti-inflammatory properties and their capacity to reduce pain (S e l a hva rzian et al, 2018; Christensen et al, 2008). In vitro studies proved that dog rose preparations are effective against certain kinds of cancer by reducing the proliferation of cancer cells (S el ahvarzi an et al, 2018)
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