Abstract
Mice treatment with pregnant mare gonadotropin serum (PMGS) in combination with human chorionic gonadotropin (hCG) is considered to be the “golden standard” of the induced superovulation procedure. The effect of administering the same doses may differ depending on the manufacturer of the preparation. According to our results, the use of PMGS produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female. At the same time, the working dose of Follimagum (Mosagrogen, Russia) was not determined. The result comparable with that of Synchrostim 500 and Sergon was achieved using a composite drug Menopur (Ferring, Germany). The study of the influence of mice age on the hormonal response showed 3–4 weeks to be the most productive age, when the maximal number of viable embryos was obtained under a minimal external invasion.
Highlights
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia 143442, Russian Federation, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
The use of pregnant mare gonadotropin serum (PMGS) produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female
The study of the influence of mice age on the hormonal response showed 3–4 weeks to be the most productive age, when the maximal number of viable embryos was obtained under a minimal external invasion
Summary
The use of PMGS produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female. Результаты и их обсуждение Подбор препаратов для стимуляции роста и развития фолликулов Для вызывания суперовуляции нами были выбраны следующие препараты ГСЖК: Фоллимаг («Мосагроген», Россия), Синхростим 500 («Ceva Sante Animale», Франция) и Сергон («Биовета», Чехия), а также комбинированные гормональные препараты Менопур («Ферринг», Германия), содержащий ФСГ и ЛГ в соотношении 1:1, и Перговерис («Мерк», Швейцария), содержащий ФСГ и ЛГ в соотношении 1:2. Сопоставимые результаты были получены в случае использования комбинированного гормонального препарата Менопур, что показывает возможность его применения для стимуляции суперовуляции у мышей вместо ГСЖК в аналогичной дозировке.
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