Optimizing the chitosan-cellulose based drug delivery system for controlling the ciprofloxacin release versus organic/inorganic crosslinker, characterization and kinetic study

Abstract

This work was done to optimize the drug delivery system based on N-trimethyl chitosan (TMC) and carboxylate-containing cellulose derivatives, as well as assessment the effective role of organic and inorganic cross-linkers for controlling release of ciprofloxacin (CPX) drug. Organic crosslinking of oxidized cellulose nanoparticle or CMC with TMC for preparing the hydrogel and their CPX drug loading were characterized by FTIR, swelling behaviour, DSC and SEM. Parallel tests were carried out on using Cu (II) ions as inorganic cross-linker.The FTIR and DSC data confirmed the formation of crosslinked delivery systems incorporated with CPX drug and candidate the TMC-CMC as the most stable delivery system. The SEM micrographs evidence the compatibility of cross-linked delivery systems with the incorporated of CPX drug through the hydrogel matrix. In vitro drug release study showed the effectiveness of organic crosslinking of TMC with CMC and OC to control the release of CPX than TMC, individually. Sustained and controlled drug releases were observed for organic crosslinked CMC (TMC-CMC) with maximum release (~75%) exceeded the TMC-OC and inorganic crosslinked CMC (Cu (II)-CMC). The release kinetics of all examined hydrogels followed Ritger-Peppas and Higuchi models, that indicating Fickian and the release of CPX was primarily controlled by diffusion process. The cell viability of human normal fibroplast cell line (BJ1) was positively correlated with the type of cellulose derivative-hydrogels and crosslinker. The TMC-CMC was recommended as promising safety and control drug release hydrogel.