Optimizing Specific Design for MPB64 in Mycobacterium tuberculosis as a Rapid Diagnosis Candidate for Pulmonary Tuberculosis

Abstract

The existing tuberculosis diagnostic with Ziehl Neelsen staining method is a main pillar in enforcing rapid and accurate diagnosis, which has low sensitivity and specificity. On the other hand, the culture method has become a gold standard, needs a longer time. This study aims to determine the specifics of MPB64 in M. tuberculosis as an alternative to the rapid diagnosis of pulmonary tuberculosis. A molecular-based cross-sectional study was conducted on 100 sputum samples of suspected TB patients. We specifically identified the variation and conserved sequence of MPB64 in M. tuberculosis in the polymorphisms. We designed a specific primer for MPB64 in M. tuberculosis conserved gene as an alternative to the rapid and accurate molecular diagnosis of tuberculosis. We found 48 positive and 52 negatives in cultures, meanwhile 35 positive and 65 negative PCR results. In the specific design of MPB64 of M. tuberculosis, we found a sequence with the primer code of F-MPTAE2 and R- MPTAE2, which is 20 bp-length. In conclusion, the specific design of MPB64 M. tuberculosis which is self-designed has potential in PCR method for M. tuberculosis diagnosis