Abstract
Hematoxylin and eosin (H&E)-stained slides inevitably deteriorate over time, frequently becoming unreadable. Reutilizing these slides can reduce the need for additional serial sections, particularly when the target region is no longer available in the tissue block. This study aims to develop efficient protocols for recycling faded H&E-stained slides, providing benefits for future research on stored samples. Seventy-one faded slides, representing a variety of tissue types and pathologies, were randomly divided into two groups. Slides were de-stained and re-stained using the conventional procedure and a modified Tris and HCl procedure. Three observers independently assessed all slides based on predefined parameters. The stability of the re-stained slides was re-assessed in 6 months. The modified Tris and HCl method yielded significantly higher scores compared to the conventional method for crispness of staining, nuclear staining, cytoplasmic staining, and vibrancy of staining (p < 0.05), as well as greater durability, as evidenced by minimal score reduction 6 months after staining. Thus, incorporating a Tris and HCl step into the process effectively enhances and restores faded H&E slides, offering a valuable technique for revitalizing histology slides for future research and educational purposes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.