Abstract
Body temperature affects outcomes of tissue injury. We hypothesized that online body core temperature recording and selective interventions help to standardize peri-interventional temperature control and the reliability of outcomes in experimental renal ischemia reperfusion injury (IRI). We recorded core temperature in up to seven mice in parallel using a Thermes USB recorder and ret-3-iso rectal probes with three different protocols. Setup A: Heating pad during ischemia time; Setup B: Heating pad from incision to wound closure; Setup C: A ventilated heating chamber before surgery and during ischemia time with surgeries performed on a heating pad. Temperature profile recording displayed significant declines upon installing anesthesia. The profile of the baseline experimental setup A revealed that <1% of the temperature readings were within the target range of 36.5 to 38.5°C. Setup B and C increased the target range readings to 34.6 ± 28.0% and 99.3 ± 1.5%, respectively. Setup C significantly increased S3 tubular necrosis, neutrophil influx, and mRNA expression of kidney injury markers. In addition, using setup C different ischemia times generated a linear correlation with acute tubular necrosis parameters at a low variability, which further correlated with the degree of kidney atrophy 5 weeks after surgery. Changing temperature control setup A to C was equivalent to 10 minutes more ischemia time. We conclude that body temperature drops quickly in mice upon initiating anesthesia. Immediate heat supply, e.g. in a ventilated heating chamber, and online core temperature monitoring can help to standardize and optimize experimental outcomes.
Highlights
Cooling reduces the cardinal signs of inflammation, i.e. dolor, calor, rubor and tumor, because temperature affects tissue perfusion, oxidative metabolism, and cytokine release
For donor hypothermia before kidney transplantation [1], for cooling organs during transport for transplantation [2], and for inducing hypothermia in patients after cardiac arrest [3]. This implies that body temperature is an important determinant for experimental ischemia reperfusion injury (IRI), a widely used method to study the pathomechanisms of acute post-ischemic injury of numerous organs including the kidney [4,5,6]
As core body temperature is a critical determinant of post-ischemic tissue injury we established the use of an online rectal temperature recording (ORTR) device to monitor body core temperature profiles during experimental IRI of the kidney using different protocols
Summary
Cooling reduces the cardinal signs of inflammation, i.e. dolor, calor, rubor and tumor, because temperature affects tissue perfusion, oxidative metabolism, and cytokine release. In this context cooling reduces the biological processes of ischemic tissue injury, which provides the rationale. For donor hypothermia before kidney transplantation [1], for cooling organs during transport for transplantation [2], and for inducing hypothermia in patients after cardiac arrest [3] This implies that body temperature is an important determinant for experimental ischemia reperfusion injury (IRI), a widely used method to study the pathomechanisms of acute post-ischemic injury of numerous organs including the kidney [4,5,6]. We speculated that standard temperature control strategies might be insufficient and report here how online body core temperature monitoring can help to stepwise optimize experimental IRI in a reliable and validated manner
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
