Optimized ultrasound-assisted extraction of total polyphenols from Empetrum nigrum and its bioactivities

Abstract

Optimization of ultrasound-assisted extraction (UAE) of total polyphenols (TPP) from Empetrum nigrum aerial parts was carried out by response surface methodology (RSM). The optimum UAE conditions of extraction time, extraction temperature, ethanol concentration, and solvent-to-material ratio were 21.38 min, 42.32 °C, 61.93% and 53.29:1 mL/g, respectively. Under the optimum conditions, the extraction yield of TPP was 32.17 ± 0.46 mg/g, which was 1.29–1.44 folds to those by the conventional extraction methods. In addition, the bioactivities of the extracts were investigated. Antioxidant activity test by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay revealed that the TPP extracts had a high potential for free radical scavenging activity. The TPP extracts showed remarkable antibacterial activity against both Gram-positive and Gram-negative strains, especially against Gram-positive strains. The evaluation of antitumor activity by the MTT assay and flow cytometric analysis indicated that the TPP extracts significantly inhibited B 16F 10 melanoma cell proliferation and effectively induced apoptosis of melanoma cells. These results demonstrate that E. nigrum aerial parts are rich in TPP and show great application potential in the pharmaceutical industry.