Optimized Synthesis of Magnesium Oxide Nanoparticles as Bactericidal Agents

Mohsen Safaei,Mohammad Moslem Imani

doi:10.1155/2019/6063832

Abstract

Increased antibiotic resistance of microorganisms as well as the need to reduce health-care costs necessitates the production of new antimicrobials at lower costs. For this reason, this study was aimed to optimize the synthesis of magnesium oxide nanoparticles with the greatest antibacterial activity. In this study, 9 experiments containing different proportions of the factors (magnesium nitrate, NaOH, and stirring time) effective in the synthesis of magnesium oxide nanoparticles were designed using the Taguchi method. Magnesium oxide nanoparticles were synthesized using the coprecipitation method, and their antibacterial activity was evaluated using colony-forming unit (CFU) and disk diffusion. Morphology, crystalline structure, and size of synthesized nanoparticles were investigated using Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM). The optimum conditions (0.2 M magnesium nitrate, 2 M NaOH, and 90 min stirring time) for the synthesis of magnesium oxide nanoparticles with the greatest antibacterial activity were determined using the Taguchi method. The results of colony-forming unit and disk diffusion revealed the optimal antibacterial activity of synthesized nanoparticles against Staphylococcus aureus and Escherichia coli bacteria. The results obtained from FTIR and XRD analyses confirmed the synthesis of nanoparticles with favorable conditions. Also, according to the SEM image, the average size of synthesized nanoparticles was determined to be about 21 nm. According to the results, magnesium oxide nanoparticles can significantly reduce the number of Gram-positive and Gram-negative bacteria and can be used as an appropriate alternative to commonly used antibacterial compounds in order to tackle drug resistance among pathogens.

Highlights

Despite significant advances in the production of drugs for the treatment of various diseases, there is still no effective treatment for some diseases, such as autoimmune diseases [1, 2], chronic pain [3], cancer [4, 5], and microbial infections [6]. e incidence of various types of microbial infections worldwide is increasing
Overuse of antibiotics and self-medication with antibiotics are common in many regions, and the use of medicines in many places is higher than global standards which have led to antimicrobial resistance among bacterial pathogens [7]. erefore, finding new solutions such as the use of modern medicine to combat the spread of antimicrobial resistance can be helpful in industry and medicine
This study aimed at optimizing the synthesis of magnesium oxide nanoparticles with the greatest antibacterial activity

Summary

Introduction

Despite significant advances in the production of drugs for the treatment of various diseases, there is still no effective treatment for some diseases, such as autoimmune diseases [1, 2], chronic pain [3], cancer [4, 5], and microbial infections [6]. e incidence of various types of microbial infections worldwide is increasing. Erefore, finding new solutions such as the use of modern medicine to combat the spread of antimicrobial resistance can be helpful in industry and medicine. Nanotechnology is mentioned as a factor which influences science and industry. The use of nanotechnology and the production of nanoparticles have created new hope for solving human problems [8, 9]. E use of nanomaterial has expanded rapidly in various fields including pharmaceutical and medical industries, which has led to the introduction of applied and novel products into the market. Many problems have been confronted in dealing with pathogenic bacteria due to emergence and spread of drug resistance among them. Many problems have been confronted in dealing with pathogenic bacteria due to emergence and spread of drug resistance among them. e increasing rates

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Conclusion