Optimized production of xylooligosaccharides from poplar: A biorefinery strategy with sequential acetic acid/sodium acetate hydrolysis followed by xylanase hydrolysis

Abstract

The preparation of xylooligosaccharides (XOS) from lignocelluloses by organic acid hydrolysis has the advantages of high efficiency and simplicity, but reducing the production of by-products, especially xylose, is a prerequisite for commercial preparation of XOS using organic acid. In this work, to reduce the production of by-products, the acetic acid/sodium acetate conjugate system (AC/SA) was used to prepare XOS from poplar. Under the optimal conditions (0.15 M AC/SA, molar ratio of 3.0, 175 °C, 60 min), the maximum XOS yield was 33.6% with a low xylose/XOS ratio of 0.19. Xylanase hydrolysis effectively converted XOS with DP above 6 in the AC/SA hydrolysate to X2-X6 with little xylose produced. The XOS yield increased to 42.1%, with a xylose/XOS ratio was only 0.17. This work shows that AC/SA in combination with xylanase hydrolysis of poplar successfully achieved high XOS yield with low by-products yields without the extraction of xylan from the substrate.