Abstract
The preparation of xylooligosaccharides (XOS) from lignocelluloses by organic acid hydrolysis has the advantages of high efficiency and simplicity, but reducing the production of by-products, especially xylose, is a prerequisite for commercial preparation of XOS using organic acid. In this work, to reduce the production of by-products, the acetic acid/sodium acetate conjugate system (AC/SA) was used to prepare XOS from poplar. Under the optimal conditions (0.15 M AC/SA, molar ratio of 3.0, 175 °C, 60 min), the maximum XOS yield was 33.6% with a low xylose/XOS ratio of 0.19. Xylanase hydrolysis effectively converted XOS with DP above 6 in the AC/SA hydrolysate to X2-X6 with little xylose produced. The XOS yield increased to 42.1%, with a xylose/XOS ratio was only 0.17. This work shows that AC/SA in combination with xylanase hydrolysis of poplar successfully achieved high XOS yield with low by-products yields without the extraction of xylan from the substrate.
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