Abstract
Optimized platform medium and feed for rCHO cell lines using the CHEF1® expression system
Highlights
Chinese Hamster Ovary (CHO) cells are widely used in biomanufacturing and biomedical research to produce proteins of clinical significance
Between Life Technologies PDDirect® and CMC Biologics, a single animal origin-free, hydrolysate-free base platform medium and three synergistic feed media were developed for use with recombinant CHO cell lines engineered using the CHEF1® expression system to produce monoclonal antibodies
Serum-free, suspension adapted CHO DG44 cells were transfected with CHEF1 plasmids harboring 2 different IgG1 MAb genes and used as test cell lines to develop a platform feed system
Summary
Chinese Hamster Ovary (CHO) cells are widely used in biomanufacturing and biomedical research to produce proteins of clinical significance. Optimized platform medium and feed for rCHO cell lines using the CHEF1® expression system One key variable in production processes is the cell culture medium used.
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