Abstract
Phenols extracted from Scirpus holoschoenus rhizomes, alone or in combination with other substances, have interesting antipseudomonal properties. Maximum extraction efficiency was achieved by using parametric optimization methods. In this study, the microwave-assisted extraction of phenolic compounds from S. holoschoenus L. rhizome was performed with the help of Response Surface Methodology, and the optimal parameters were found to be 56% acetone, 69s time and 600W for power, with a TPC value of 30.70±1.22mg GAE/g dry weight (dw). The ethyl acetate fraction (EA) of the optimized extract was tested in combination with diluted essential oil of T. fontanesii (EOd) and lactic acid (LA) on Pseudomonas aeruginosa. Mixture design indicated that at 7°C, the 75% EOd, 25% EA and 0% LA mixture gave the largest diameter inhibition zone (63±2mm). A mixture of 83.7% EOd and 16.28% EA was the most effective at 37°C (24.16±1.04mm).
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