Abstract
An optimized method for the extraction and quantification of artemisinin using high-performance thin-layer chromatography (HPTLC) from dried Artemisia annua L. leaf was established. Seven solvents with different polarities were used for the efficient extraction of artemisinin by hot Soxhlet as well as by conventional soaking method at room temperature. Among these solvents, n-hexane was found to be the superior solvent for both extraction methods. The chromatographic separation was carried out on precoated silica gel plate G60 F254 using n-hexane-ethyl acetate-acetic acid (2:1:0.1) as the mobile phase, and densitometric analysis was carried out in absorbance mode at 540 nm after derivatization with anisaldehyde spraying reagent. The HPTLC method was validated for specificity as well as for recovery. The optimized mobile phase gave well-defined peaks of artemisinin at Rf value of 0.43 ± 0.006. The linear regression analysis of the calibration plots using standard artemisinin exhibited good linear relations...
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