Abstract
We used an accurate and green technique “Dispersive Liquid Liquid Micro Extraction” (DLLME) followed by HPLC-UV analysis for the analysis of benzoates (BA), sorbates (SA), and p-hydroxyl methyl benzoates (MB) in fruits products. We optimized the extraction parameters by the box Behnken design. We validated the method by using the β accuracy profile including the systematic (trueness) and random (intermediate precision) errors. The extraction efficiency varied from 82.98 to 100.8 %. This method was linear with R2s values higher than 0.9752 for all the compounds. The repeatability and intermediate precision were less than 3.18 and 12.02 respectively; whereas, the detection limits were respectively 0.304, 0.306 and 0.153 mg/ ml for BA, SA and MB. This method is therefore reliable to the simultaneous quantification of these preservatives in fruit juices, nectars and jams. The levels of these additives in the samples exceeded 833.97 and 290.67 ppm for BA and SA, respectively; while, MB was not detected.
Highlights
Preservatives such as Benzoic acid, sodium and potassium benzoates, sorbic acid and sorbates, methyl para hydroxyl benzoates or their salts are used widely in different industrial sectors such as pharmaceuticals, cosmetics and food [1,2,3,4]
This graph was drawn according to the total error obtained from the sum of the bias and the intermediate precision for each level and takes into account the expected proportion of acceptable results comprised between predefined acceptability limits or expresses the probability of measurements that are expected to fall outside the acceptance limits during routine analysis. This approach allows minimizing considerably the risk to accept a procedure, which is not sufficiently accurate, or to reject a procedure giving accurate measures. The aim of this present work is the development, optimization and validation of a dispersive liquid-liquid micro extraction (DLLME) technique followed by an HPLC-UV analysis
3.1.1 Selection of the extracting and dispersive solvents The extractive and dispersive solvents are essential parameters in DLLME development. These two solvents are the most relevant factors which affect the efficiency of this technique. The choice of these solvents is based on the following criteria: the extracting solvent was selected after a previously screening of different solvents according to their water miscibility, affinity with the analytes of interest and high yield of extraction and high sensitivity, with higher density than water to facilitate its recovery
Summary
Preservatives such as Benzoic acid, sodium and potassium benzoates, sorbic acid and sorbates, methyl para hydroxyl benzoates or their salts are used widely in different industrial sectors such as pharmaceuticals, cosmetics and food [1,2,3,4]. These compounds are used to extend the shelf life of food products because of their large spectrum activity against bacteria, yeasts, and moulds [4,5,6]. According to Bledzka [12], these compounds are detected in breast tissues, suggesting the possibility of their involvement in breast cancer The monitoring of these additives in fruit-based products is essential to protect consumers’ health from toxicological effects
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