Abstract
The effects of sucrose onDaucus carota cell size and biosynthesis of carotenoid were investigated. At high concentration of sucrose, the growth rate and the final cell mass were low due to the substrate inhibition, and cell size was small compared to the result at low sucrose concentration. However, very high level of specific carotenoid content was obtained from small sized cells compared to the condition from large sized cells. The biosynthesis of carotenoid can be increased by optimizing the cell culture method as follow: (i) cultivate the cell initially at low sucrose concentration to increase the cell growth rate, and (ii) when cell mass reaches a certain level, increase the sucrose concentration to a high level to make the cell size small and to increase the biosynthesis of carotenoid.
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