Optimization studies for extraction of antioxidants from Mucuna sanjappae seeds: A promising natural drug for oxidative stress management

Abstract

Introduction and Aim: Cellular oxidative stress is responsible for various human diseases. Dietary antioxidant supplements from natural plants have good potential to reduce increased oxidative stress thus protecting from further medical complications. Genus Mucuna is well known for its promising health benefits. A common species of Mucuna, M. pruriens is being used for oxidative stress related diseases treatment. However, many species of this genus are not scientifically explored for their health-related beneficial properties. In the present study, we have made effort to investigate secondary metabolites composition and antioxidant potential in a Mucuna species namely M. sanjappae. In this work, we tried to understand superior system for secondary metabolites (total phenolics and flavonoids content) extraction and antioxidant activity in M. sanjappae seeds was demonstrated. Methodology: Four different solvents viz., water, methanol, ethanol, and acetone in combination with four different extraction conditions (static, shaking, microwave and sonication) each with three different times have been studied. Total 48 extract samples were generated which were used for total phenolics, total flavonoids content along with antioxidant activity (DPPH, DMPD and FRAP) determination. Further major phenolics were identified using HPLC system. Results: Water showed highest TPC and TFC level during microwave method for 120 seconds (81.627±0.728 mg GAE g-1 and 648.06±4.37 mg RUE g-1 respectively). The highest antioxidant activity was also found in M. sanjappae seeds extracted in water. RP-HPLC quantification of phenolics revealed tannic acid and gallic acid as predominant compounds. Correlation analysis indicated that the antioxidant of different extracted samples demonstrated a good positive relationship with TPC and TFC. Conclusion: Present attempt indicated that, simple solvent water along with microwave method can be used for maximum antioxidants extraction from M. sanjappae seeds. This Mucuna species may be further studied to exploit as a natural antioxidant source in medicine and food etc.