Abstract
Micropropagation of orchids are often through the production of Protocorm Like Bodies (PLBs) and this research conducted a study to discover the optimal condition for the growth of Protocorm Like Bodies (PLBs) and also plantlets regeneration of Grammatophyllum speciosum Several aspects such as different carbon sources (sucrose, maltose) and the presence of charcoal alongside with the variety of concentration of agar were taken into account for the optimization. This study discovered that the 30 g/L of sucrose in 1 mg/L of BAP was superior in producing heavy PLBs. However, the trend differs in the number of plantlets where maltose (5 g/L) is seen to produce a higher number of plantlets compared to sucrose. Charcoal plays an important role in the growth of PLBs where the heaviest PLBs were recorded in the medium supplemented with 0.20 % of charcoal along with 4.5 g of agar. Vice versa relationship was recorded for the number of plantlets where without charcoal in 4.5 g of agar produce the highest number of plantlets.
Highlights
Grammatophyllum speciosum can be categorized under the family of Orchidaceae, which contains a diverse range of lowering plants with over 100,000 hybrids resulted from arti icial pollination Cardoso et al (2020)
A similar trend can be seen for 5 mg/L of BAP where maltose (10 g/L) recorded the lowest reading for the weight of Protocorm Like Bodies (PLBs) (8.1g) among other carbon sources supplemented with 5 mg/L of BAP
Sopalun et al (2010) recorded maltose produced the highest relative growth of PLBs of tiger orchid but the concentration of maltose used in the study was only 2 % and the sucrose used in the study was only 2 %
Summary
Grammatophyllum speciosum can be categorized under the family of Orchidaceae, which contains a diverse range of lowering plants with over 100,000 hybrids resulted from arti icial pollination Cardoso et al (2020). Classi ied as the largest orchid in the world, Grammatophyllum speciosum is commonly known as tiger orchid and is native to Malaysia, Indonesia, the Philippines, and Thailand. A single tiger orchid plant can produce up to 7000 lowers in a season, it only blooms once every two to four years Chee (2015). Micropropagation is one of the initiatives to continue producing tiger orchids throughout the whole year. How to cite this article (APA): Rahman, Z. Optimization parameter for production of protocorm-like body and growth of grammatophyllum speciosum. International Journal of Research - GRANTHAALAYAH, 9(7), 145-154.
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