Abstract
The continuous growth of the biomedical market highlights the significance of improvement in the efficiency of batch production for protein drug crystals. Herein, we investigated the effect of polymer-modified Fe3O4 nanoparticles on lysozyme (Lys) crystallization at different conditions, using functionalized Fe3O4 magnetic nanoparticles (Polymer C@Fe3O4) as nucleation agents. The study revealed that the surface charge density of Polymer C@Fe3O4 was highest at a modification mass ratio of Fe3O4 to Polymer C is 8:3. This ratio facilitates the augmentation of electrostatic interaction and the promotion of crystallization. By adding 15% of the PolymerC@Fe3O4 prepared from Fe3O4 with an average particle size of 150 nm, the magnetic core lysozyme (M-Lys) crystals with an average particle size of 11.08 μm, narrow size distribution, and regular morphology were obtained in a yield of 82.42% within 60 min.
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