Abstract
The focus of wastewater management has evolved from treatment technology into resource recovery, which enables one to minimize contaminants and to generate value-added products. Calvatia gigantea is used not only as a source of food, but has also been used as a traditional Chinese medicine for thousands of years. In this study, the mycelial production of C. gigantea was studied under submerged fermentation conditions using non-pretreated distilled wastewater from Chinese liquor production. The fermentation medium composition was optimized using response surface methodology involving a Box–Behnken design. Fermentation conditions were optimized using an orthogonal experimental design. The optimized medium composition was the non-pretreated distilled wastewater of Chinese liquor supplemented with cornflour at 2.35 g/100 mL, (NH4)2SO4 at 1.11 g/100 mL and CuSO4 at 0.12 g/100 mL. The optimized fermentation conditions were a rotation speed of 150 rpm, an inoculum size of 10% (v/v), a fermentation temperature of 26 °C and a fermentation time 4.5 days. A maximum mycelial biomass yield of 2.75 g/100 mL was achieved using the optimized medium under the optimized conditions. Results from this study suggest that this is a feasible technology for the mycelial production of C. gigantea using the non-pretreated distilled wastewater from Chinese liquor production. Copyright © 2015 The Institute of Brewing & Distilling
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