Abstract
Abstract In the present study, rapid eco-friendly method was applied to optimize and synthesize zinc oxide nano particles from Crateva adansonii using Box-Behnken Design (BBD) of response surface methodology (RSM). The best optimal conditions for enhanced green synthesis of ZnONP from Crateva adansonii were ZnNO3 (3 mM), incubation time (15 h), temperature (65 ᵒC) and pH (6).Green synthesized and optimized ZnONP exhibits a strong absorption peak at 292 nm in UV region and it shows FTIR absorption spectrum at 536 cm−1 is due to the characteristic absorption of Zn-O bond. Green synthesized ZnONP shown hexagonal wurtzite structure and 30–55 nm in size reveals significant antimicrobial activity against human clinical pathogens. ZnONP showed maximum zone of inhibition 18 mm and 16 mm against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. Eco-friendly green synthesized Zinc oxide nanoparticles from Crateva adansonii shows significant antimicrobial activity against human clinical pathogens and it useful to treat multi drug resistance bacterial pathogens.
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