Optimization of xylose production from wheat straw using of chemicals and enzymatic catalases

Abstract

Wheat straw is one of the most abundant agricultural by product and it is an attractive source of lignocelluloses , which is a renewable resource for the production of fuels and chemicals, and therefore their exploitation could be economically feasible. Biotechnological conversion of biomass into fuels and chemicals requires hydrolysis of the polysaccharide fraction into monomeric sugars. In this study, the effects of various pretreatments ( concentration different of NaOH, H 2 SO 4 ) as well as solid to liquid ratio, reaction temperature and time and subsequent enzymatic hydrolysis of wheat straw using autoclave processing which is essential to hydrolysis of wheat straw for xylose release were examined. The results of hydrolysis degree indicated that the optimal reaction conditions for the recovery of xylose from wheat straw hemicellulose were obtained when autoclave wheat straw was treated with xylanase, ground wheat straw was autoclaved 10 parts water at 121 ◦ C and the liquid and solid phases separated. The maximum xylose concentration was 23.38 mg/mL at optimized reaction conditions of 40°C reaction temperature, 30 min. reaction time and liquid-solid ratio of 1:10 (volume-mass). DOI = 10.24897/acn.64.68.53