Abstract
Hydrolytic enzymes play a significant role in hydrolyzing lignocellulosic polysaccharides into fermentable sugars. Due to the complex structure of lignocellulosic materials, developing robust processes for high titer production is a major challenge. The present study was investigated on xylanase production by filamentous fungi Aspergillus fumigatus strain JCM 10253 using agricultural residue ragi husk as a substrate for microbial fermentation. Central composite design (CCD) was employed to optimize the independent process variables and their effect on xylanase activity. Experimental results with optimized process variables showed 156.7 IU/mL activity under 1.24% substrate concentration and pH 2.0 at 49.9 °C after 120 h of the incubation period. The morphological study of isolate JCM 10253 was observed by scanning electron microscopy analysis and Fourier transform infrared spectroscopy was used to characterize the crude enzyme for secondary protein helical structures. Thus, this study demonstrates that ragi husk could be a potential substrate for xylanase production and can be further used as value-added industrial products.
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