Abstract
An efficient ultrasound-assisted extraction (UAE) technique was applied to extract total phenolic content (TPC) from yarrow (Achillea beibrestinii). A response surface methodology was employed to evaluate the effects of 4 independent variables (temperature, pH, solvent to sample ratio, and time) on the TPC. The optimum extraction conditions were as follows: liquid to solid ratio 20, pH 6.3, extraction temperature 35°C, and extraction time 35 min. Under the above-mentioned conditions, the experimental TPC was 11.7 mg gallic acid equivalent/g dry matter. EC50 of the UAE extract at optimal conditions was found at 30.6±0.6 mg/L level and total flavonoids (TF) were obtained at 12.6±0.2 mg/10 g sample. Also, the results of HPLC analysis showed that gallic acid and p-coumaric acid were the predominant phenolic compounds in the plant.
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