Abstract

In this work, an ultrasound-assisted procedure for the extraction of defatted wheat germ proteins by reverse micelles was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction (UAE) parameters (ultrasonic output power, ultrasonic time and pulse mode) for enhancing the forward extraction efficiency of defatted wheat germ proteins by implementing a three-level, three-variable Box–Behnken experimental design. The independent variable with the largest effect on response was X 2 (ultrasonic time), followed by X 1 (ultrasonic output power) and X 3 (pulse mode). The optimum extraction conditions were found to be ultrasonic output power 363 W, ultrasonic time 24 min and pulse mode 2.4 s on and 2 s off (2.4 s:2 s). Under these conditions, the forward extraction efficiency of defatted wheat germ proteins can increase from 37% to 57%, and the final protein extraction efficiency by reverse micelles can reach 45.6%, making the advantage of reverse micelles much more obvious than alkaline extraction and isoelectric precipitation on the separation of protein.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.