Abstract
The optimization of antioxidant extraction conditions from a ripe edible fruits of Zizyphus lotus (L.) with an ultrasound-assisted system was achieved by response surface methodology. The central composite rotatable design was employed for optimization of extraction parameters in terms of total phenolic content and antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and phosphomolybdenum assay. The optimum operating conditions for extraction were as follows: ethanol concentration, 50%; extraction time, 25min; extraction temperature, 63°C and ratio of solvent to solid, 67mL/g. Under these conditions, the obtained extract exhibited a high content of phenolic compounds (40.782mg gallic acid equivalents/g dry matter) with significant antioxidant properties (the total antioxidant activity was 75.981mg gallic acid equivalents/g dry matter and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 0.289mg/mL).
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