Optimization of ultrasonic‐assisted extraction and purification of flavonoids with biological activities from Ficus Pumila L.

Abstract

AbstractTo optimize the extraction process and activity detection of total flavonoids from Ficus Pumila, a Box–Behnken design of response surface methodology was employed to further optimize ultrasonic‐assisted extraction (UAE) conditions for F. Pumila stems total flavonoids (FPSTF). The results showed that optimized extraction conditions were ultrasonic power of 240 W, solvent concentration of 61%, ultrasonic time of 61 min, and liquid‐solid ratio of 25 mL/g. Under these conditions, the mean experimental value of extraction yield (1.07%) was achieved, which was about 2.14 times that of ethanol leaching extraction. As compared with FPSTF, FPSTF after purification (FPSPTF) had higher purity of 11.09%, which was nearly 4.37 times higher than that of FPSTF(2.54%). As compared with FPSTF, FPSPTF had higher α‐glucosidase and α‐amylase inhibitory activities as well as DPPH· and ABTS·+ scavenging activities with an IC50 value of 0.005, 0.008, 0.22, and 0.08 mg/mL, which was 6.00, 16.50, 1.68, and 2.50 times higher than that of FPSTF (0.030, 0.132, 0.37, and 0.20 mg/mL), respectively. FPSPTF from F. Pumila could be considered as a multifunctional bioactive ingredient to be used in anti‐hyperglycemic pharmaceutical formulation and antioxidant.