Abstract
Previous study only implemented the time consuming and low amount of yield technique for extraction from Chromolaena odorata which is conventional method. Nonconventional extraction method with short extraction time and high amount of yield was applied in this study by applying ultrasound-assisted enzymatic extraction (UAEE). UAEE was used to extract tannic acid from Chromolaena odorata. The extraction parameters involved were enzyme concentration, sonication time and duty cycle at constant temperature of 50°C, solid to liquid ratio of 1:10 and sonication power at 60% amplitude. The optimum extraction process was found at cellulase enzyme concentration of 4%, sonication time of 60 minutes and duty cycle of 50% with the obtained concentration of tannic acid at 1.6152 mg/mL. The study showed that the UAEE could be employed to enhance yield of tannic acid, reduce the extraction time and ensuring green extraction method were applied in the study.
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